JRC GMO-Amplicons | EURL GMFF

Record ID:	MW432177.1
Database:	nt	Go to original record
Description:	Dolichandrone spathacea chloroplast, complete genome.
Taxon:	Dolichandrone spathacea
Original size:	159139 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47788	48351	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt ggctgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgaag gataaacgta tctattgaat actatatcaa atgattaatg
301   atggcccgaa tctgtatctg tattttttta tatgaaacat ggaagaatta
351   gtgtgaattg attccacatt gaagaaagaa tcgaatattc attcatcaaa
401   tcattcactc cacagtccga tagttctttt aaagaactga ttaagcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details