JRC GMO-Amplicons | EURL GMFF

Record ID:	MW432176.1
Database:	nt	Go to original record
Description:	Dolichandrone cauda-felina chloroplast, complete genome.
Taxon:	Markhamia cauda-felina
Original size:	159538 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47316	47880	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg gctgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcgcccga atctgtatct gtattttttt atatgaaaga tggaagaatt
351   agtgtgaatt gattccacat tgaagaaaga atcgaatatt cattcatcaa
401   atcattcact ccatagtccg atagttcttt taaagaactg attaagcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details