JRC GMO-Amplicons | EURL GMFF

Record ID:	MW464332.1
Database:	nt	Go to original record
Description:	Impatiens cyanantha chloroplast, complete genome.
Taxon:	Impatiens cyanantha
Original size:	152375 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46716	47310	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tggaaaacaa atccaaataa
151   agattccgaa accaaaaata aaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tggtagagaa atccgtggat
251   cgaaacttca gaaagtatga aaaaaacata tatacatatg catatatgct
301   gaaatactat atcaatagta tatcaaaata aaatgaatga cgatcaaaaa
351   gcgtatcctt ttttttttag taaaaaaatg gttgtgaatc gattccatat
401   tgaataaaga atcaaatatt cattggtcaa atcatttact ccaaagtgtg
451   atagttcctc tgaagaactg attaatcaga cgagaataaa gatagagtcc
501   cattatacat gtcaatcccg acaacaatga aatttatagt aataggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details