JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860642.1
Database:	nt	Go to original record
Description:	Veratrum oxysepalum tRNA-Leu (trnL) gene, intron.
Taxon:	Veratrum oxysepalum
Original size:	601 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	601	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gagccttggc aaggaaacct gctaagtgat
51    aacttccaaa ttcagggaaa ccctggaaaa aaaagggggc aatcctgagc
101   caaatcttga ttttgagaaa tcaagggttt aggtttagtt tctaaaatca
151   aactagaata aaaaaagata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatggagt tgactacgtt gcgttgatcg ctggaattcc tctatggaaa
251   ttaaagaaag gatggcccac atatatttta tacatatnct ttatatatat
301   atatactgac atatcgatcg attaatcatg acccgaatcc atattatata
351   ttatataaaa tatatatgaa aaaggaagag ttattgtgaa tccattccaa
401   tcgaagttaa gggaagaatc gaatattcag tgatcaaatc attcattcca
451   aagtctaata gatcttttga aaaaggattc ataggacgag aataaagaga
501   gagtcccatt atacatgtca ataccgacaa caatgaaatt tatagtaaga
551   ggaaaatacc gtcgacctta aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details