JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860641.1
Database:	nt	Go to original record
Description:	Vaccinium vitis-idaea tRNA-Leu (trnL) gene, intron.
Taxon:	Vaccinium vitis-idaea
Original size:	572 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	572	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aaaaaaaaat
101   gggcaatcct gagccaaatc cttttttttt tggcaaacaa ataaagattc
151   cgaaagaaaa aaaaagggat aggtgcagag actcaatgga agctattcta
201   acaaatggag ttgactgcgt tggtagaaag aataggaatt ctttcatcga
251   aactttcgaa aagatgaaag ataactctat atacatacgc ataggtaatg
301   attaataaca accaaaatct gtatccctat ttttttatat gaaaaataga
351   caaatttttg tgaattgatt tcacattgaa taaagattta catatttatt
401   gatcaaatca tttactccat catctgctag ttcttttgaa gaactgatga
451   atcgcacgag aataaagata gagtcccatt ctacatgtca ataccgacaa
501   gaatgaaatt tgcttgtaag aggaaaatcc gtcgacttta gaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details