JRC GMO-Amplicons | EURL GMFF

Record ID:	MW774642.1
Database:	nt	Go to original record
Description:	Callitriche palustris chloroplast, complete genome.
Taxon:	Callitriche palustris
Original size:	150138 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45608	46179	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ttcaaaacaa aggttctaaa
151   aacgaaatta agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgtgttggt agaggaatct ttacatcaaa acttcagaaa
251   ggatgaagga taaacgtatc tattgaatac tctataaaat aaaatatata
301   taaaatagat aatggtgact tgagtcttta tctttttttt tatatgaaaa
351   aatggaagaa ttggtgtgaa ttgattccaa ttgaaaaaac aatctaatat
401   tcatcaaatt actcactcca tagtctgata gatcttttaa acaactgatt
451   aattagacga gaataaagat agagtcccat tctacatgtc aataccgaca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgatttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details