JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860639.1
Database:	nt	Go to original record
Description:	Vaccinium alaskaense tRNA-Leu (trnL) gene, intron.
Taxon:	Vaccinium ovalifolium
Original size:	569 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	569	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaat
101   gggcaatcct gagccaaatc cttttttttt ttggcaaaca aataaagatt
151   ccgaaagaaa aagggatagg tgcagagact caatggaagc tattctaaca
201   aatggagttg actgcgttgg tagaaagaat aggaattctt tcatcgaaac
251   tttcgaaaag atgaaagata actctatata catacgcata ggtaatgatt
301   aataacaacc aaaatctgta tccctatttt tttatatgaa aaatagacaa
351   atttttgtga attgatttca cattgaataa agatttacat atttattgat
401   caaatcattt actccatcat ctgctagttc ttttgaagaa ctgatgaatc
451   gcacgagaat aaagatagag tcccattcta catgtcaata ccgacaagaa
501   tgaaatttgc ttgtaagagg aaaatccgtc gactttagaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details