JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860636.1
Database:	nt	Go to original record
Description:	Tofieldia pusilla tRNA-Leu (trnL) gene, intron.
Taxon:	Tofieldia pusilla
Original size:	595 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	595	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttgattg gcttgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttgttttga taaaacaagg ttttctaaaa
151   ctagaatcaa aaggggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg cattggtagt cggaatcctc atatattatg
251   gaaaggatag gatagcctta tatacgtaat atatacgtat acgtactgac
301   atatcaaacg attaatcacg acccgaatcc agaatttcta taaattctat
351   gaaatgcaaa atttcagagt tatttggaat ccattctaat ccaagttgat
401   tgaaggaaga atcgaatatt cagtgatcaa accattcact ccagagtctg
451   attgatcttt tgaaaaaatg atgaatcgga caagaataaa gagagagtcc
501   cattctacat gtcaatatcg acaacaatga aatttatagt aagaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details