JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860635.1
Database:	nt	Go to original record
Description:	Tofieldia coccinea tRNA-Leu (trnL) gene, intron.
Taxon:	Tofieldia coccinea
Original size:	590 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttgattg gcttgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttgttttta taaaacaaga ttttataaaa
151   ctagaatcaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg cattggtagt cggaatcctc atatattgtg
251   gataggatag ccttatatac ctaatatata cgtatacata ctgacatatc
301   aaacgattaa tcacgacccg aatccataat ttatggaaat tctacgaaat
351   gcaaaatttt agagttattt ggaatccatt ctaatccaag ttgattgaag
401   gaagaatcga atattcagtg atcaaaccat tcactccaga gtctgattga
451   tcttttgaaa aaatgatgaa tcggacaaga ataaagagag agtcccattc
501   tacatgtcaa tatcgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details