JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860634.1
Database:	nt	Go to original record
Description:	Thalictrum alpinum tRNA-Leu (trnL) gene, intron.
Taxon:	Thalictrum alpinum
Original size:	601 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	601	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttgattg ggttgagcct taattatgga
51    aacatactaa gtgataactt tcaaattcag agaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctggtttca gaaaacaaaa aaggggttca
151   gaaagcaaga atacaaaaag aaaaggatag gtgcagagac tcaatggaag
201   ctgttctaac gaatagagtt gactgctttg atcgaggaat gaatccttct
251   atttttaaaa ctccagaaag gatgaaccca tatacgtaca taatacgtaa
301   taaaatacca aagattaatc cgaatcttta ttttttattt tctatatatg
351   tatgttatat gcaaaattga agaattcttg tgaatcgact ccaagtttaa
401   gtaagtatcg aatactcgct gatcaaatca gtcactccat agtctgatag
451   atctttcttt taaagaacta actaattgga cgagaataaa gatagagtcc
501   cattatacat gtcaatatca ataccggcaa aaatgaaatt tatagtaaga
551   ggaaaatccg tcgacttttg aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details