JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860625.1
Database:	nt	Go to original record
Description:	Saxifraga setigera tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga setigera
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    accttactaa gtgataactt tcaaattcag ggaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctattttaa gaaaacaaaa cccaaaggtt
151   cagaaagaga gaataaaaaa ggataggtgc agagactcaa tggaagctat
201   tctaacaaag ggagttgact gcgatgcggt ggtaaaagaa tccgtccctc
251   gaaacttcag aaaggctgaa ggctaaactt tttttcgttt tattattatg
301   tatacggact gaaattctta aaaaaaaatc aaattattaa tgacggctca
351   agtctttttt ttttcaacta aaaaaattgt tgtaaatcga ttacaagttg
401   aagaaagaat tgaatattca ttgataaaac cattcacgtc atagtctgat
451   aaatcctttg aagagctgat taatcaaaca agaataaaga tagagtccca
501   ttctacatgt caatgctgac agcaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt ataaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details