JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860624.1
Database:	nt	Go to original record
Description:	Saxifraga radiata tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga radiata
Original size:	607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	607	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaagcaacac acaagggttc
151   agaaaaagcg aaaaaagaat aggataggtg cagagactca atggaagcta
201   ttctaacaaa gggagttgac tgcgatgcgg tggtaaaaga atccttccat
251   cgaaacttca gaaaggctga aggctaaact tttatacata ttattattat
301   attattatgt ctacgtactg aaatattata taaaaatcaa atgattaata
351   ttaatgacga ctcaagtctt ttttttttcg ttgaaaaaaa ttgttctaaa
401   tagattacaa gttgaagaaa gaattgcata ttcattgata aaaccattca
451   cgctatagtc tgataaatcc tttgaagagc tgattaatca gacaagaata
501   aagatagagt cccattctac atgtcaatgc cgacagcaat gaaatttata
551   gtaagaggaa aatccgtcga cttaagaaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details