JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860623.1
Database:	nt	Go to original record
Description:	Saxifraga oppositifolia tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga oppositifolia
Original size:	598 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaacgg aaaagagccc cggtatggaa
51    acctacgaag tgataacttt caaattcagg gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaagcaaaac acaagggttc
151   agaaaaagcg aataaaaaaa aggataggtg cagagactca atggaagcta
201   ttctaacaaa gggagttgac tgcgatgcgg tgctaaaaga atccttccat
251   cgaaacttca gaaaggctga aggctaaact tttatacata ttattatgta
301   tacgtactga aatattatat aaaaatcaaa tgattaatat taatgatgac
351   tcaagtcttt tttttttttc aacgaaaaaa ttgttgtaaa tcgattacaa
401   gttgaagaac gaattgaata ttcattgata aaaccattca cgctatagtc
451   tgataaatcc tttgaagagc tgattaatca gacaagaata aagatagagt
501   cccattctac atgtcaatac cgacagcaat aaaatttata gtaagaggaa
551   aatccgtcga cttttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details