JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860620.1
Database:	nt	Go to original record
Description:	Saxifraga hirculus tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga hirculus
Original size:	607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	607	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaaacaaaac acaagggttc
151   cgaaagagag aataaaaaag gataggtgca gagactcaat ggaagctatt
201   ctaacaaagg gagttgactg cgatgcggtg gtaaaagaat ccttccatcg
251   aaacttcaga aaggctaaag gctaaacttt tatacgtatt attattatgt
301   atacgtactt aaatactata taaaaataaa aaagaaaatt attaatgacg
351   actcaagtct tttttttcaa ctaaaaaaat tgttgtaaat cgattacaag
401   ttgaagaaag aattgaatat tcattgataa aaccattcac gtcatagtct
451   gataaatcct ttgaagagct gattaatcat taatcagaca agaaagaata
501   aagatagagt cccattctac atgtcaatgc tgacagcaat gaaatttata
551   gtaagaggaa aatccgtcga ctttataaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details