JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860619.1
Database:	nt	Go to original record
Description:	Saxifraga flexuosa tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga flexuosa
Original size:	597 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtaggaaa
51    cctactaagt gataactttc aaattcaggg aaaccctgga attaaaaatg
101   ggcaatcctg agccaaatcc tgttttaaga aagcaacaca caaaaaaagc
151   gaaaaaagaa taggataggt gcagagactc aatggaagct attctaacaa
201   agggagttga ctgcgatgcg gtggtaaaag aatccttcca tcgaaacttc
251   agaaaggctg aaggctaaac ttttatacat attattatta tattattatg
301   tatacgtact ggaatattat ataaaaatca aatgattaat attaatgacg
351   actcaagtct ttttttttcg ttgaaaaaaa ttgttctaaa tagattacaa
401   gttgaagaaa gaattgcata ttcattgata aaaccattca cgctatagtc
451   tgataaatcc tttgaagagc tgattaatca gacaagaata aagatagagt
501   cccattctac atgtcaatgc cgacagcaat gaaatttata gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details