JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860618.1
Database:	nt	Go to original record
Description:	Saxifraga cespitosa tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga cespitosa
Original size:	585 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaagcaaaac acaagggttc
151   agaaaaagaa aaaaaaaaag gataggtgca gagactcaat ggaagctatt
201   ctaacaaagg gaattgactg tgatgcggtg gtaaaagaat ccttccatcg
251   aaacttcaga aaggctgaag gctaaacttt tatacatatt atttattatg
301   tatacgtact gaaatattat ataaaaataa aatgataaat ttaatattaa
351   ttaatattaa tgacgactca agtctttttt ttcaacgaaa aaattgttgt
401   aaatcgatta taagttgaag aaagaattga atattcattg ataaatcctt
451   tgaagagctg attaatcaga caagaataaa gatagagtcc cattctacat
501   gtcaatgctg acagcaatga aattaatagt aagaggaaaa tccgtcgact
551   ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details