JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860617.1
Database:	nt	Go to original record
Description:	Saxifraga cernua tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga cernua
Original size:	606 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	606	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaagcaacac acaagggttc
151   agaaaaagcg aaaaaagaat aggataggtg cagagactca atggaagcta
201   ttctaacaaa gggagttgac tgcgatgcgg tggtaaaaga atccttccat
251   cgaaacttca gaaaggctga aggctaaact tttatacata ttattattat
301   attattatgt atacgtactg aaatattata taaaaatcaa atgattaata
351   ttaatgacga ctcaagtctt tttttttcgt tgaaaaaaat tgttctaaat
401   agattacaag ttgaagaaag aattgcatat tcattgataa aaccattcac
451   gctatagtct gataaatcct ttgaagagct gattaatcag acaagaataa
501   agatagagtc ccattctaca tgtcaatgcc gacagcaatg aaatttatag
551   taagaggaaa atccgtcgac tttagaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details