JRC GMO-Amplicons | EURL GMFF

Record ID:	MT507771.1
Database:	nt	Go to original record
Description:	Pseudocerastium stellarioides voucher X. H. Guo 951054 (ANUB) plastid, complete genome.
Taxon:	Pseudocerastium stellarioides
Original size:	148741 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44918	45515	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaatag gtttgagcct tagtatggaa
51    acctgctaag tgaaaacttt caaattcaga gaaaccctgg aaaaaaaagg
101   gcaatcctga gccaactcct taattgtctc ctaaaaaaaa acggattcaa
151   aaagaataaa aaaggatagg tgcagagact caaaggaagc tgttctaaaa
201   aaaatgggga cgtcctgaaa tgattaatga caacccgaat ttgttctgta
251   ttttttttta cttaattata ttatataatt atattataat taatattaat
301   aataatatat ataatctcta gaaatccata ttataggata gatagattta
351   aaatatggaa aaatgcaaga attgttatgg atcgattcta agttgaaagc
401   cgaataaatc tttgagttat tcataaaaac attcatacta accccatagt
451   ctgaaagatc ttttgaagaa gggattaatc ggatgagaat aaagatagag
501   tcccgttcta catgtcaata ttgacaacaa ttaaatttat agtaagagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details