JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860616.1
Database:	nt	Go to original record
Description:	Saxifraga bronchialis tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga bronchialis
Original size:	608 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	608	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttaatta aaaaagagcc ttggtatgga
51    aacctactaa gtgataactt tcaaattcag ggaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctgttttaa gaaaacaaaa cacaagggtt
151   cagaaataaa aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaatctaac aaagggagtt gactgcgatg cggtggtaaa agaatccttc
251   catcgaaact tcagaaaggc tgaaggataa acttttatac atattattat
301   ggatacgtac tgaaatacta tatcaaaatc aaatgattaa tgacgactca
351   agtctttatt tatttttttt ttcaacgaaa aaaattgttg taaatcgatt
401   acaagttgaa gaaagaattg aatcttcatt gataaaacca ttcacgccat
451   agtctgataa atcctttgaa gaactgatta attgattaat cagacaagaa
501   taaagataga gtcccattct acatgtcaat gccgacagca atggggttgg
551   gttgtaagga ggaaaatccg tcggctttta aatcgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details