JRC GMO-Amplicons | EURL GMFF

Record ID:	MW783683.1
Database:	nt	Go to original record
Description:	Veronica undulata voucher 20200709 chloroplast, complete genome.
Taxon:	Veronica undulata
Original size:	151178 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46237	46804	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctaaaaacaa agattcagaa
151   agttaaaaaa aggggatagg tgcagagact cgatggaagc tgttctaaca
201   aatggagtta gttgactgta tcggtagatg aatcgtttca tcgaaacttc
251   agaaagaatg aaggataaac ggatctattg aattgaatac tatataaaat
301   aaaaaactta atggtgggcc gagtctgtat ctgtattttt tttttaatac
351   aagacttggt gtaaattgat tccacattca agaaagaatc gaatattcat
401   aaaatcaccc actccatagt ctgatagttc ttttaaagaa ctgattaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ccgacaaaaa
501   tgaaatttct agtaagagga aaatccgtcg aatttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details