JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860615.1
Database:	nt	Go to original record
Description:	Saxifraga bracteata tRNA-Leu (trnL) gene, intron.
Taxon:	Saxifraga bracteata
Original size:	610 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	610	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttaag aaagcaacac acaagggttc
151   agaaaaagcg aaaaaagaat aggataggtg cagagactca atggaagcta
201   ttctaacaaa gggagttgac tgcgatgcgg tggtaaaaga atccttccat
251   cgaaacttca gaaaggctga aggctaaact tttatacata ttattattat
301   attatattat tatgtatacg tactgaaata ttatataaaa atcaaatgat
351   taatattaat gacgactcta gtcttttttt ttcgttgaaa aaattgttct
401   aaatagatta caagttgagg aaagaattgc atattcattg ataaaaccat
451   tcacgctata gtctgataaa tcctttgaag agctgattaa tcagacaaga
501   ataaagatag agtcccattc tacatgtcaa tgccgacagc aatgaaattt
551   atagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details