JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860605.1
Database:	nt	Go to original record
Description:	Rumex acetosa tRNA-Leu (trnL) gene, intron.
Taxon:	Rumex acetosa
Original size:	540 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	540	+	540 bp

Amplicon:

540 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtacggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   tgggcaatcc tgagccaact cctcctttcc aaaaggaaga ataaaaaagg
151   ataggtgcag agactcaatg gaagctgttc taacaaatgg agttgaggat
201   cttgcgttat tagttattat aagaattctt ccatcgaaac tccaaaaagg
251   atgaagaata aacctatacg cagacatact taaatataaa tactatataa
301   ctataactag ttattatatt tagttattct attttatata tttatataat
351   tttatataat aaaaaataat aaattaaata aagcatttat ttgaagtctg
401   atagaccttt tgaagatcca tcgtatgaga atgaagatag agtcccattc
451   tacgtgtcaa tcttgacacc actgaaattt atagtaagaa gaaaatccgt
501   cgactttata cgtcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	540	521	2.43e-08

Match details