JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860603.1
Database:	nt	Go to original record
Description:	Rubus arcticus tRNA-Leu (trnL) gene, intron.
Taxon:	Rubus arcticus
Original size:	567 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	567	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acatactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtttttat aaaacaaatc aaaatcaaat
151   aaagggttca gaaagcaaga ataaaaaagg ataggtgcag agactcaatg
201   gaagctgttc taacgaatag agttgactgc gttgatcgag gaatccttct
251   atttaaactc tagaaaggat gaacccatat acgtacatat acgtaataaa
301   atttcaaaga aagattcata tatgttatat gcaaaattga agaattcttg
351   tgaatcgatt ctaagtttaa ggaagaatcg aatattcact gatcaaatca
401   gtcactccat agtctgatag atcttttaaa gaactaacta attggacgag
451   aataaagata gagtcccatt atacatgtca atatcaatac cgacaaaaat
501   gaaatttata gtaagaggaa aatccgtcga ctttttaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details