JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860602.1
Database:	nt	Go to original record
Description:	Rhododendron tomentosum tRNA-Leu (trnL) gene, intron.
Taxon:	Rhododendron tomentosum
Original size:	585 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aaaaaaaaat
101   gggcaatcct gagccaaatc cttttttcgc aaacaaacaa agattccgaa
151   agctaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaatag aaatccttct atcgaaactt
251   acgaaaaaat gaaagataat tctatataca tacgtatagg cactgaaata
301   ctatataaaa tgattaataa cgaccaaaat ctgtatccct attttttttt
351   ataaaaaaaa tggaccaatt ctcgtgaatt tatttcacat tgaataaaaa
401   attgcatatt tattgatcaa atcatttact ccatcgtcta ctagatcttt
451   tgaagatctg atgaatcgga tgagaataaa gatagagtcc cattctacat
501   gtcaataccg acaacaatga aatttattgt aagaggaaaa tccgtcgact
551   ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details