JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860600.1
Database:	nt	Go to original record
Description:	Ranunculus sulphureus tRNA-Leu (trnL) gene, intron.
Taxon:	Ranunculus sulphureus
Original size:	587 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	587	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acatactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgctttcag aaaacaaaaa aaaaggggat
151   tcagaaagcg aaaataggga taggtgcaga gactcaatgg aagctgttct
201   aacgaataga gttgactgcg ttgatcgagg aatccttcta tttgaattcc
251   cgaaaggatg aaccaatcca tatacgtacg tacaaatatg taataaaata
301   ggaaagaagc aacccaaatc tttatttttt atgttatatg caaaacaaat
351   agaagaattc ttgtgaatcg attctaggtt gaagtaagaa tcgaatattc
401   aatattcatt gatcaaatca gttattctct aacctggtag attttttaaa
451   gaactgactg gttggacgag aataaagata gagtcccatt atacatgtca
501   atatcaatac cgacaaaaat gaaatttata gtaagaggaa aatccgtcga
551   cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details