JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860599.1
Database:	nt	Go to original record
Description:	Ranunculus pygmaeus tRNA-Leu (trnL) gene, intron.
Taxon:	Ranunculus pygmaeus
Original size:	577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	577	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tgatatggaa
51    acatactaag gataactttc aaattcagag aaaccctgga ataaaaaatg
101   ggcaatcctg agccaaatcc tgctttcaga aaaaaaaaaa agggattcag
151   aaagcgaaaa tagggatagg tgcagagact caatggaagc tgttctaacg
201   aatagagttg actgcgttga tcgaggaatc cttctatttg aattcccgaa
251   aggatgaacc aatccatata cgtacgtaca aatatgtaat aaaataggaa
301   agaagcaacc caaatcttta ttttttatgt tatatgcaaa acaaatagaa
351   gaattcttgt gaatcgattc taagttgaag taagaatcga atattcaata
401   ttcattgatc aaatcagtta ttctctaacc tggtagattt tttaaagaac
451   tgactggttg gacgagaata aagatagagt cccattatac atgtcaatac
501   cgacaaaaat gaaatttata gtaagaggaa aatccgtcga cttttaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details