JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860598.1
Database:	nt	Go to original record
Description:	Ranunculus nivalis tRNA-Leu (trnL) gene, intron.
Taxon:	Ranunculus nivalis
Original size:	584 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	584	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tgatatggaa
51    acatactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgctttcag aaaaaaaaaa aaagggattc
151   agaaagcgaa aatagggata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatagagt tgactgcgtt gatcgaggaa tccttctatt tgaattcccg
251   aaaggatgaa ccaatccata tacgtacgta caaatatgta ataaaatagg
301   aaagaagcaa cccaaatctt tattttttat gttatatgca aaacaaatag
351   aagaattctt gtgaatcgat tctaagttga agtaagaatc gaatattcaa
401   tattcattga tcaaatcagt tattctctaa cctggtagat tttttaaaga
451   actgactggt tggacgagaa taaagataga gtcccattat acatgtcaat
501   atcaataccg acaaaaatga aatttatagt aagaggaaat ccgtcgactt
551   ttaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details