JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860597.1
Database:	nt	Go to original record
Description:	Pyrola grandiflora tRNA-Leu (trnL) gene, intron.
Taxon:	Pyrola grandiflora
Original size:	579 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	579	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aaaaaaaaat
101   gggcaatcct gagccaaatc cttttttttt ttgaaatttt tgtttgtttt
151   cgaaaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact acgttggtag agaaatccga aacttacgat acgaaaagat
251   gcaagatagc tctatataca tacgcataca tactgaaata ctatatcaac
301   ttattaataa cgattaaaat ttgtatcctt attttttttt tgaaaaatga
351   acgaattgat gtgaatccat ttttcatttt acattgaata aagaattgca
401   tattttttga tcaaatcatt tcctccatag tctgataaat cttttgaaga
451   actgattaat cagacgagaa taaagataga gtcccattct acatgtcaat
501   actgacaaca atgaaattta tagtaagagg aaaatccgtc gactttagaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details