JRC GMO-Amplicons | EURL GMFF

Record ID:	MK558819.1
Database:	nt	Go to original record
Description:	Plantago sp. JP-2021 chloroplast, complete genome.
Taxon:	Plantago sp. JP-2021
Original size:	164946 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45873	46455	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactagg tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   aaaatgggca atcctgagcc aaatcctgtc ttctcaaaat aaaggttcat
151   aaagcgaaaa ggggataggt gcagagactc gatggaagct gttctaaaac
201   aaatggagtt gactgcgttg gtagatgaat cttttcatcg aaacttcaga
251   aaagatgaag gataaacgga tctattgaat actaaaatat ctaaaaaaaa
301   ggaatgacgg cccgagtctg catctgtatt tttttagatg aaaaatagaa
351   gaattggtgg gaattgattc cacattcaag aaagaatcga atattcattc
401   atcaaatcac cccactccat agtctgatag ttttagtctt tttttaaaga
451   actgattaat taattggacg agaataaaga tagagtcccg ttctacatgt
501   caataccgac agcaatgaaa tttatagtaa taggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details