JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860596.1
Database:	nt	Go to original record
Description:	Puccinellia wrightii tRNA-Leu (trnL) gene, intron.
Taxon:	Puccinellia wrightii
Original size:	613 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	613	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggga
51    aacctgctaa gtggtaactt ccaaattcag agaaaccctg gaattaaaaa
101   agggcaatcc tgagccaaat ccgtgttttg agaaaacctg ggggttctcg
151   aactagaata caaaggaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat cgagttaatt acgttgtgtt gttagtggaa ttccttctaa
251   attcgagaag gaagggcttt atacatctaa taaacaccta tagatactgc
301   catagcaaac gattaatcac agaacccata ttataatata ggttctttat
351   tcttttttag aatcaaatag aaatgattat gaaataaaaa attcataatt
401   tttttagaat tattgtgaat ccattcccat ccgaatattt agtaatcaaa
451   tccttcaatt caaagttttg aggtctttaa aaaagtagat taatcgaacg
501   agttcaaaga gcgagtccca ttctacatgt caatactgac aacaatgaaa
551   tttttagtaa aaggaaaatc cgtcggcttt ttaagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details