JRC GMO-Amplicons | EURL GMFF

Record ID:	MW599988.1
Database:	nt	Go to original record
Description:	Clerodendranthus spicatus chloroplast, complete genome.
Taxon:	Clerodendranthus spicatus
Original size:	152155 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46907	47452	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tgggagttga ctgcgctggt agaggaatct ttccatggaa actttagaaa
251   ggatgaagga taaacgcatc tattgaatct atatcaaatg gttaatgatg
301   gcccgaatct tttaatatga aaaaatggaa aaattggtgt gaattgattc
351   tacgttgaag aaaaaatcga atattcatca actcattcgc tccatagtcc
401   gatagatctt ttaaagaact gattaatcgg acgagaataa agatagagtc
451   ccattctaca tgtcaatatc ggcaacaatg aaatttatag taagaggaaa
501   atccgtcgac tttaaaaatc gtgagggttc aagtccctct atcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details