JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860594.1
Database:	nt	Go to original record
Description:	Primula nutans tRNA-Leu (trnL) gene, intron.
Taxon:	Primula nutans
Original size:	592 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgggaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggtaat cctgagccaa atcctctttt tagaaaacaa agatgaaaag
151   aaagggggat aggtgcagag actcaatgga agctgttcta acaaatggag
201   ttggtcgcgt tgctagagga atcctttcat gaaaatttca gaaaggatgc
251   aaagataaat gtatatatat atgcatatgt gcatatgtac tgaaatacta
301   tatcaaaata aaatgtttat ttttttctat gaattttttc tatgaaaaaa
351   aaatagaaga attgttgcga atcgattcta cattcgacat tgactaaata
401   atttcatatt cattgatcaa agcatttaat ttactccata gtctgataga
451   tcttgcgaat aactgattaa tcaggcgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aataatgaaa tttatagtaa taggaaatcc
551   gtcgacttta gaaaccgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details