JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860593.1
Database:	nt	Go to original record
Description:	Primula anvilensis tRNA-Leu (trnL) gene, intron.
Taxon:	Primula anvilensis
Original size:	614 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgggaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcctctttt tagaaaacaa agatgaaagg
151   aaagggggga taggtgcaga gactcaatgg aagctgttct aacaaatgga
201   gttggtcgcg ttgccagagg aatcctttca tgaaaatttc agaaaggatg
251   caaatataaa tgtatataca tatgcatatg tgcatatgta ctgaaatact
301   atatcaaaat caaattttta tttttttcaa tgtttctttt tttctatgaa
351   tttttttcta tgaaaaaaaa ataaaaaaat agaagaattg ttgcgaatcg
401   attctacatt ccacattgac taaataattt catattcatt gatcaaagca
451   tttactccat agtctgatag atcttgcgaa taactgatta atcaggcgag
501   aataaagata gagtcccatt ctacatgtca ataccgacaa caatagtgca
551   atttatagta agcggaaaaa tccgtcgact tttaaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details