JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860591.1
Database:	nt	Go to original record
Description:	Potentilla uniflora tRNA-Leu (trnL) gene, intron.
Taxon:	Potentilla uniflora
Original size:	603 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccgttttatg aaaacaaaca agggtttcat
151   aaagcgagaa taaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc attgtgttca taaaggaatc cttccatcga
251   aacttccgaa aagatgaaag ataaacctat atacatatgt atacttacgg
301   atgtatactt actgaaatac tatcgccaaa tgattaaaaa tgattaatga
351   cgactccaac cggttctata atttttttct atctatttat atgatagaaa
401   aaaaaagaat taaatattca ttgatcaaaa cattcactcc atcatagtcc
451   gataaatctt tttattttga agaatttttt aatcggatta agaataaaga
501   tagagtccca ttttacatgt caatatcgac aacaaagaaa tttatagtaa
551   gaggaaaatc cgtcgatttt agaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details