JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860590.1
Database:	nt	Go to original record
Description:	Potentilla pulchella tRNA-Leu (trnL) gene, intron.
Taxon:	Potentilla pulchella
Original size:	594 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccgttttatg aaaacaaaca agggtttcat
151   aaagcgagaa taaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc attgtgttca taaaggaatc cttccatcga
251   aacttccgaa aagatgaaag ataaacctat atacatatgt atacttacgg
301   atgtatactt actgaaatac tatcgccaaa tgattaaaaa tgattaatga
351   cgactccaac cggttctata atttatttat atgatagaaa aaaaaaagaa
401   ttaaatattc attgatcaaa acattcactc catcatagtc cgataaatct
451   ttttattttg aagaattttt taatcggatt aagaataaag atagagtccc
501   attttacatg tcaatatcga caacaaagaa atttatagta agaggaaaat
551   ccgtcgattt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details