JRC GMO-Amplicons | EURL GMFF

Record ID:	MN604379.1
Database:	nt	Go to original record
Description:	Dysosma versipellis chloroplast, complete genome.
Taxon:	Dysosma versipellis
Original size:	156735 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49127	49667	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactgag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcgg aaaagggata ggtgcagaga
151   ctcgatggaa gctgttctaa cgaatgaagt tggtcgaaga aaatctttct
201   attaaaactc cagaaaggat gaacctatat atgatatata ggtatacgta
251   ctgaaataaa atagaaaaga ttaatgaaat acgaatcctt tatttaactt
301   atacaaaaat tgaaaaattt ttctgaatcg attccatgtg gaagtaagga
351   tcgaatattc actgatcaaa tcagccactt tagagtatag tatgatatag
401   attttgtgaa gaactaacta attggacgag aataaagata gagtcccatt
451   ctacatgtca ataccgacaa caatgaaatt tatagtaaga ggaaaatccg
501   tcgactttag caatcgtgag ggttcaagtc cctctatccc c

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details