JRC GMO-Amplicons | EURL GMFF

Record ID:	MT119357.1
Database:	nt	Go to original record
Description:	Parapholis strigosa plastid, partial genome.
Taxon:	Parapholis strigosa
Original size:	136895 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48171	48784	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaaggggt tctcgaacta
151   gaatacaaag gaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatcgagt taattacgtt ttgttgttag tggaattctt tctaaattcg
251   agaaagaagg gctttataca tctaataaac acgtatagat actgacatag
301   caaacgatta atcacagaac ccatattcta atataggttc tttattcttt
351   tttagaatga aattggaaat agaaatgatt ctgaaataaa aaattcataa
401   tttttttaga attattgtga atccattcca atcgaatatt tagtaatcaa
451   atccttaaat tcaaagtttt gaggtcttta aaaaagcgga ttaatcggac
501   gaggacaaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagttgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details