JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860586.1
Database:	nt	Go to original record
Description:	Polemonium acutiflorum tRNA-Leu (trnL) gene, intron.
Taxon:	Polemonium acutiflorum
Original size:	594 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttattt tcgaaaacaa acaaagattc
151   ggaaagcaaa aataagaaaa aaaaaggggg gataggtgca gagactcaac
201   ggaagctgtt ctaacaaatg gatttgactg cgttggtcaa ggaatctttt
251   catcgaaact taagaaagga tgaaagaaaa acatatatac atatgcatac
301   gtactgaaat actatatcaa tttattaata acgacctaaa cctgtatccg
351   tatttttttc tataaaaaaa gaaagaagtg ttgtgaatag attccacaat
401   gaataaagaa ttgaatgctc gttgatcaaa taagttaatc catagtctga
451   tagatctttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   gttctacatg tcaataccga cagcaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details