JRC GMO-Amplicons | EURL GMFF

Record ID:	MW348925.1
Database:	nt	Go to original record
Description:	Myoporum bontioides chloroplast, complete genome.
Taxon:	Myoporum bontioides
Original size:	151719 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48032	48594	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggatgaagg ataaaggtat atattgaata ctatatcaaa tgattaatga
301   tggccctaat ctgtatctgt atttttttat atgaaaaatg gaagaattgg
351   tatgaagtga ttccacattg aagaaagaat caaatattcc ttcatcaaat
401   cattcactcc acagtctgat agttctttta aagaactgat taatcggaca
451   agaataaaga tagagtccca ttctacatgt caatattggc aacaatgcaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaagtcgtg agggttcaag
551   tccctctatc ccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details