JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860580.1
Database:	nt	Go to original record
Description:	Pedicularis oederi tRNA-Leu (trnL) gene, intron.
Taxon:	Pedicularis oederi
Original size:	566 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	566	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattt aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaggggcaat cctgagccaa atcttctttt tttttcaaaa caagggttca
151   gaaaacgaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg attacactga tagaggaatg aaacttcaga aaagatgaag
251   gataaacgta tctattgaat actatatcaa atgattgatt aatcatggcc
301   caaatctgta tctgtatttt tgaatacaga aaagtggaat aatttgtgtg
351   aattgattcc atattgaaga aagaatatta attcatcaaa tcattcactc
401   caaagtccga tagatctttt taaaaagatc tgattaaatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccggca ataatgaagt
501   ttatagtaaa aggaaaattc cgtgcgggct tttttaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details