JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860579.1
Database:	nt	Go to original record
Description:	Pedicularis langsdorffii tRNA-Leu (trnL) gene, intron.
Taxon:	Pedicularis langsdorffii
Original size:	559 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	559	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagctt tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaggggcaat cctgagccaa atcttctttt ttttttcaaa acaaaggttt
151   agaaaacgaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgattacgct gatagaggaa tgaaacttca gaaaagatga
251   aggataaacg tatctattga atactatatc aaatgattga ttaatcatgg
301   cccaaatctg tatctgtatt tttgaataca gaaaagtgga agaatttgtg
351   tgaattgatt ccatattgaa gaaagaatat taattcatca aatcattcac
401   tccatagtcc gatagatctt tttaaaaaga tctgattaat tggacgagaa
451   taaagataga gtcccattct acatgtcaat accggcaata atgaaatttt
501   tagtaaaagg aaaatccgtc gacttttaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details