JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860578.1
Database:	nt	Go to original record
Description:	Pedicularis albolabiata tRNA-Leu (trnL) gene, intron.
Taxon:	Pedicularis albolabiata
Original size:	557 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	557	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagctt tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aggggcaatc ctgagccaaa tcttcttttt tttttcaaaa caaaggtttc
151   gaaaacgaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg attacgctga tataggaatg aaacttcaga aaagatgaag
251   gataaacgta tctattgaat actatatcaa atgattgatt aatcatggcc
301   caaatctgta tctatatttt tgaatacaga aaagtggaag aatttgtgtg
351   aattgattcc atattgaaga aagaatatta attcatcaaa tcattcactc
401   catagtccga tagatctttt taaaaagatc tgattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaatac cggcaataat gaaattttta
501   gtaaaaggaa aatccgtcga cttttaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details