JRC GMO-Amplicons | EURL GMFF

Record ID:	MT504973.1
Database:	nt	Go to original record
Description:	Heteropogon contortus voucher K:3209 chloroplast, complete genome.
Taxon:	Heteropogon contortus
Original size:	134979 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47701	48226	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttta aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac aaagaaccca tattataata
251   taggttcttt atttttagaa tgaaattagg aatgattatg aaatagaaaa
301   ttcataattt tttgagaatt aattattgtg aatctattcc aatcgaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatcct tttaaaagcg
401   gattaatcgg acgaggataa agagagagtc ccattctaca tgtcaatact
451   gacaacaatg aaatttctag taaaaggaaa atccgtcgac tttataagtc
501   gtgagggttc aagtccctct atcccc

View other 20 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details