JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860577.1
Database:	nt	Go to original record
Description:	Parrya nudicaulis tRNA-Leu (trnL) gene, intron.
Taxon:	Parrya nudicaulis
Original size:	585 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctggtttacg cgaactaacc agagtttata
151   aagggagaaa aagggatagg tgcagagact caacggaagc tgttctaaca
201   aatggagttc actaccttgt gttgataagg gaatccttcg atcgaaactg
251   aaaatcaaaa cggatgaagg agaaaaacct attttgtcta aaaataggta
301   acacaaaacg atctaaaaaa tgacgacctg aatctctatt ctatatttct
351   attaaaaaaa aatagaaatg ttgtgaatca attcgaagtt taagaaaaaa
401   tcgaatattc attgaatcaa atgattcact tcatagtctg atagatcctt
451   ggtggaactt gttaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaatactg acaacaatga aatttatagt aagatgaaaa tccgttgact
551   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details