JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860572.1
Database:	nt	Go to original record
Description:	Oxytropis maydelliana tRNA-Leu (trnL) gene, intron.
Taxon:	Oxytropis maydelliana
Original size:	619 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	619	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaacaaaaa aataaaagtt
151   cagaaagtta aaatgaaaca aataaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgacaacat ttcctttcgc agtaggaaat
251   taatccttct atcaaaattc tagaaaggat caaagataaa catatatata
301   tatatatata tatatttact gaaatactat ttcagttgat taatgaaaat
351   tacaaactta tatttgtaaa tatttagatt agattcgatt aaaaaagatg
401   tgaatcaaat caattccaac ttgaagaaaa aatggaatat tcattgatca
451   aatcagtcac tccaccatag tctgatggat cttttgaaga aatgattaat
501   cagacgagaa taaagataga gtcccattct acatgtcaat accgacacca
551   atgaaatttt tagtaagagg aaaatccgtc gactttagaa atcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details