JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860571.1
Database:	nt	Go to original record
Description:	Oxytropis campestris tRNA-Leu (trnL) gene, intron.
Taxon:	Oxytropis campestris
Original size:	605 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	605	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaacaaaaa aataaaagtt
151   cagaaagtta aaatgaaaca aataaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgacaacat ttcctttcgc agtaggaaat
251   taatccttct atcaaaattc tagaaaggat caaagataaa catatatata
301   tttactgaaa tactatttca gttgattaat gaaaattaca aacttatatt
351   tgtaaatatt tagattagat tcgattaaaa aagatgtgaa tcaaatcaat
401   tccaacttga agaaaaaatg gaatattcat tgatcaaatc agtcactcca
451   ccatagtctg atggatcttt tgaagaaatg attaatcaga cgagaataaa
501   gatagagtcc cattctacat gtcaataccg acaccaatga aatttttagt
551   aagaggaaaa tccgtcgact ttagaaatcg tgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details