JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860570.1
Database:	nt	Go to original record
Description:	Oxytropis borealis tRNA-Leu (trnL) gene, intron.
Taxon:	Oxytropis borealis
Original size:	621 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	621	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaacaaaaa aataaaagtt
151   cagaaagtta aaatgaaaca aataaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgacaacat ttcctttcgc agtaggaaat
251   taatccttct atcaaaattc tagaaaggat caaagataaa catatatata
301   tatatatata tatatattta ctgaaatact atttcagttg attaatgaaa
351   attacaaact tatatttgta aatatttaga ttagattcga ttaaaaaaga
401   tgtgaatcaa atcaattcca acttgaagaa aaaatggaat attcattgat
451   caaatcagtc actccaccat agtctgatgg atcttttgaa gaaatgatta
501   atcagacgag aataaagata gagtcccatt ctacatgtca ataccgacac
551   caatgaaatt tttagtaaga ggaaaatccg tcgactttag aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details