JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860569.1
Database:	nt	Go to original record
Description:	Oxyria digyna tRNA-Leu (trnL) gene, intron.
Taxon:	Oxyria digyna
Original size:	618 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	618	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aagaaaaaaa
101   tgggcaatcc tgagccaact ccttcgtcca aaaggaagaa taaaaaagga
151   taggtgcaga gactcaatgg aagctgttct aacaaatgga gttgacgatc
201   ttgcgttatt ataagataag aattcttcca tctaaactct aaaaaggatt
251   aagaataaac atatatacat atgcngacgt agtgaaatat aaatactata
301   agaatataac ataactagtt attacataaa aagatttaat tttaaaatat
351   tgatgacgac ccgcattttt tttatgactc ggaaaaaatg gaagaattgt
401   tgtgaaatga ttccaagttg aagaaagaat cgaatattcc tttaataaat
451   taaagcattt attccccggt ctgatagatc ttttgaagaa ccggtccatc
501   ggatgagaat gaagatagag tcccattcta cgtgtcaatc ctgacaacaa
551   tgaaatttat agtaagagga aaatccgtcg actttataaa tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details