JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860563.1
Database:	nt	Go to original record
Description:	Juncus arcticus tRNA-Leu (trnL) gene, intron.
Taxon:	Juncus arcticus
Original size:	614 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acttgctaag tagtaacttc caaattcaga ggaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaattggt ttttatagaa
151   aaactcaaat caaaaaagat aggtgcagag actcaacgga agctattcta
201   acgaataaag ttgactacgt tacattggta gttggaattt ctctagggaa
251   cgaaataagt cgaaataaga taaaagatga tcctatatat actaaaatca
301   tacgtatata tactgattaa tcaaatgatt aatcataata aggatccaat
351   tctattctgc atcttttgaa tgaaattatg aatgatttat tatgaaatag
401   taaattcaga aaaaataaat tatgttgaat gaaaggagaa tccaagattc
451   agtaatcaaa aaatgcattc ccgagtttgc tgaaaaactg atgaatgggg
501   cgagaataaa gagagagtcc attctacatg tcaatatcga caacaatgaa
551   atttatagta agaagaaaat ccgtcgattt taggaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details