JRC GMO-Amplicons | EURL GMFF

Record ID:	MK341052.1
Database:	nt	Go to original record
Description:	Zygophyllum fabago chloroplast, complete genome.
Taxon:	Zygophyllum fabago
Original size:	104984 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46965	47505	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaaaaa agaattcaat
151   caaaagaatc aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   aaaaatggag gactgtgcat taagttagtc aagtcaagga atccttgcat
251   caaaactttc aaaaagatga aggataacct ttctttctat aaaactataa
301   aaagaaaaag actttttgaa ttaattgaat caaatcaatt ggaagttgaa
351   gaaagaatca aatatgattt gatcaaataa tttactccaa agtctgatat
401   atctttttca aaatagatta gtcagatgag aataaagata gagtcccatt
451   ctatatgtca gtattgacaa caatgaaatt tatagtgaga ggaaaatccg
501   tcgattttat aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details